Integrin α5 Is Regulated by miR-218-5p in Endothelial Progenitor Cells.

ABSTRACT:

Background

Endothelial cell injury is a common nidus of renal injury in patients and consistent with the high prevalence of AKI reported during the coronavirus disease 2019 pandemic. This cell type expresses integrin α5 (ITGA5), which is essential to the Tie2 signaling pathway. The microRNA miR-218-5p is upregulated in endothelial progenitor cells (EPCs) after hypoxia, but microRNA regulation of Tie2 in the EPC lineage is unclear.

Methods

We isolated human kidney-derived EPCs (hkEPCs) and surveyed microRNA target transcripts. A preclinical model of ischemic kidney injury was used to evaluate the effect of hkEPCs on capillary repair. We used a genetic knockout model to evaluate the effect of deleting endogenous expression of miR-218 specifically in angioblasts.

Resu

SUBMITTER: Liu J 

PROVIDER: S-EPMC8975065 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature