Formation of recombinant bifunctional fusion protein: A newer approach to combine the activities of two enzymes in a single protein.

ABSTRACT: The tissue of insects, pests, and fungi has a chitin layer followed by protein in the cell membrane. The complete biodegradation of chitin and protein-present in the waste requires the action of two enzymes, namely chitinase, and protease. Combining chitinase and protease in a single protein/enzyme will serve as a bifunctional enzyme that can efficiently degrade the chitin and protein-rich biomass. The present study was aimed to fuse these two enzymes to produce a single protein and study the kinetics of the recombinant fusion protein. A chitinase and alkaline protease genes were isolated, cloned, and expressed successfully as a fusion product in heterologous host Escherichia coli. The two native genes were successfully fused in E.coli by using flexible glycine-serine (G4S)2 linker (GGGGS,

SUBMITTER: Nilpa P 

PROVIDER: S-EPMC8975109 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature