Robust and annotation-free analysis of alternative splicing across diverse cell types in mice.

ABSTRACT: Although alternative splicing is a fundamental and pervasive aspect of gene expression in higher eukaryotes, it is often omitted from single-cell studies due to quantification challenges inherent to commonly used short-read sequencing technologies. Here, we undertake the analysis of alternative splicing across numerous diverse murine cell types from two large-scale single-cell datasets-the Tabula Muris and BRAIN Initiative Cell Census Network-while accounting for understudied technical artifacts and unannotated events. We find strong and general cell-type-specific alternative splicing, complementary to total gene expression but of similar discriminatory value, and identify a large volume of novel splicing events. We specifically highlight splicing variation across different cell typ

SUBMITTER: Benegas G 

PROVIDER: S-EPMC8975553 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature