Real-time and automated monitoring of antischistosomal drug activity profiles for screening of compound libraries.

ABSTRACT: Schistosomiasis is a neglected tropical disease that affects over 200 million people annually. As the antischistosomal drug pipeline is currently empty, repurposing of compound libraries has become a source for accelerating drug development, which demands the implementation of high-throughput and efficient screening strategies. Here, we present a parallelized impedance-based platform for continuous and automated viability evaluation of Schistosoma mansoni schistosomula in 128 microwells during 72 h to identify antischistosomal hits in vitro. By initially screening 57 repurposed compounds against larvae, five drugs are identified, which reduce parasite viability by more than 70%. The activity profiles of the selected drugs are then investigated via real-time dose-response moni

SUBMITTER: Ravaynia PS 

PROVIDER: S-EPMC8976133 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature