Identification of chromatin states during zebrafish gastrulation using CUT&RUN and CUT&Tag.

ABSTRACT:

Background

Cell fate decisions are governed by interactions between sequence-specific transcription factors and a dynamic chromatin landscape. Zebrafish offer a powerful system for probing the mechanisms that drive these cell fate choices, especially in the context of early embryogenesis. However, technical challenges associated with conventional methods for chromatin profiling have slowed progress toward understanding the exact relationships between chromatin changes, transcription factor binding, and cellular differentiation during zebrafish embryogenesis.

Results

To overcome these challenges, we adapted the chromatin profiling methods Cleavage Under Targets and Release Using Nuclease (CUT&RUN) and CUT&Tag for use in zebrafish and applied these methods to generate high-res

SUBMITTER: Akdogan-Ozdilek B 

PROVIDER: S-EPMC8976701 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature