A suspension technique for efficient large-scale cancer organoid culturing and perturbation screens.

ABSTRACT: Organoid cell culture methodologies are enabling the generation of cell models from healthy and diseased tissue. Patient-derived cancer organoids that recapitulate the genetic and histopathological diversity of patient tumours are being systematically generated, providing an opportunity to investigate new cancer biology and therapeutic approaches. The use of organoid cultures for many applications, including genetic and chemical perturbation screens, is limited due to the technical demands and cost associated with their handling and propagation. Here we report and benchmark a suspension culture technique for cancer organoids which allows for the expansion of models to tens of millions of cells with increased efficiency in comparison to standard organoid culturing protocols. Using whole-gen

SUBMITTER: Price S 

PROVIDER: S-EPMC8976852 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature