Development and immunohistochemical characterization of patient-derived xenograft models for muscle invasive bladder cancer.

ABSTRACT:

Objectives

Patient-derived xenograft (PDX) models have become a valuable tool to evaluate chemotherapeutics and investigate personalized cancer treatment options. The role of PDXs in the study of bladder cancer, especially for improvement of novel targeted therapies, continues to expand. In this study, we aimed to establish autochthonous PDX models of muscle-invasive bladder cancer (MIBC) to provide a useful tool to conduct research on personalized therapy.

Materials and methods

Tumors from MIBC patients undergoing radical cystectomy were subcutaneously transplanted into immunodeficient mice. The tumor size was measured by a caliper twice a week for up to five months. After the first growth in mice, they were serially passaged. Hematoxylin and eosin (H&E) staining and immuno

SUBMITTER: Razzaghdoust A 

PROVIDER: S-EPMC8976898 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature