Monocarboxylate Transporter 1 (MCT1) Mediates Succinate Export in the Retina.

ABSTRACT:

Purpose

Succinate is exported by the retina and imported by eyecup tissue. The transporters mediating this process have not yet been identified. Recent studies showed that monocarboxylate transporter 1 (MCT1) can transport succinate across plasma membranes in cardiac and skeletal muscle. Retina and retinal pigment epithelium (RPE) both express multiple MCT isoforms including MCT1. We tested the hypothesis that MCTs facilitate retinal succinate export and RPE succinate import.

Methods

We assessed retinal succinate export and eyecup succinate import in short-term ex vivo culture using gas chromatography-mass spectrometry. We tested the dependence of succinate export and import on pH, proton ionophores, conventional MCT substrates, and the MCT inhibitors AZD3965, AR-C155858, an

SUBMITTER: Bisbach CM 

PROVIDER: S-EPMC8976921 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature