Immune-instructive copolymer scaffolds using plant-derived nanoparticles to promote bone regeneration.

ABSTRACT:

Background

Age-driven immune signals cause a state of chronic low-grade inflammation and in consequence affect bone healing and cause challenges for clinicians when repairing critical-sized bone defects in elderly patients.

Methods

Poly(L-lactide-co-ɛ-caprolactone) (PLCA) scaffolds are functionalized with plant-derived nanoparticles from potato, rhamnogalacturonan-I (RG-I), to investigate their ability to modulate inflammation in vitro in neutrophils and macrophages at gene and protein levels. The scaffolds' early and late host response at gene, protein and histological levels is tested in vivo in a subcutaneous rat model and their potential to promote bone regeneration in an aged rodent was tested in a critical-sized calvaria bone defect. Significant differences were tested

SUBMITTER: Suliman S 

PROVIDER: S-EPMC8977008 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature