Elevated ASCL1 activity creates de novo regulatory elements associated with neuronal differentiation.

ABSTRACT:

Background

The pro-neural transcription factor ASCL1 is a master regulator of neurogenesis and a key factor necessary for the reprogramming of permissive cell types to neurons. Endogenously, ASCL1 expression is often associated with neuroblast stem-ness. Moreover, ASCL1-mediated reprogramming of fibroblasts to differentiated neurons is commonly achieved using artificially high levels of ASCL1 protein, where ASCL1 acts as an "on-target" pioneer factor. However, the genome-wide effects of enhancing ASCL1 activity in a permissive neurogenic environment has not been thoroughly investigated. Here, we overexpressed ASCL1 in the neuronally-permissive context of neuroblastoma (NB) cells where modest endogenous ASCL1 supports the neuroblast programme.

Results

Increasing ASCL1 in neur

SUBMITTER: Woods LM 

PROVIDER: S-EPMC8977041 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature