In vitro culture of ovine embryos up to early gastrulating stages.

ABSTRACT: Developmental failures occurring shortly after blastocyst hatching from the zona pellucida constitute a major cause of pregnancy losses in both humans and farm ungulates. The developmental events occurring following hatching in ungulates include the proliferation and maturation of extra-embryonic membranes - trophoblast and hypoblast - and the formation of a flat embryonic disc, similar to that found in humans, which initiates gastrulation prior to implantation. Unfortunately, our understanding of these key processes for embryo survival is limited because current culture systems cannot sustain ungulate embryo development beyond hatching. Here, we report a culture system that recapitulates most developmental landmarks of gastrulating ovine embryos: trophoblast maturation, hypoblast migratio

SUBMITTER: Ramos-Ibeas P 

PROVIDER: S-EPMC8977095 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature