Evaluation of porcine urine-derived cells as nuclei donor for somatic cell nuclear transfer.

ABSTRACT:

Background

Somatic cell nuclear transfer (SCNT) is used widely in cloning, stem cell research, and regenerative medicine. The type of donor cells is a key factor affecting the SCNT efficiency.

Objectives

This study examined whether urine-derived somatic cells could be used as donors for SCNT in pigs.

Methods

The viability of cells isolated from urine was assessed using trypan blue and propidium iodide staining. The H3K9me3/H3K27me3 level of the cells was analyzed by immunofluorescence. The in vitro developmental ability of SCNT embryos was evaluated by the blastocyst rate and the expression levels of the core pluripotency factor. Blastocyst cell apoptosis was examined using a terminal deoxynucleotidyl transferase dUTP nick end-labeling assay. The in vivo

SUBMITTER: Zhang YT 

PROVIDER: S-EPMC8977534 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature