Coupling nucleic acid circuitry with the CRISPR-Cas12a system for universal and signal-on detection.

ABSTRACT: We report a universal and signal-on HCR based detection platform via innovatively coupling the CRISPR-Cas12a system with HCR. By using this CRISPR-HCR pathway, we can detect different targets by only changing the crRNA. The CRISPR-HCR platform coupling with an upstream amplifier can achieve a practical sensitivity as low as ∼aM of ASFV gene in serum.

SUBMITTER: Zhao R 

PROVIDER: S-EPMC8977996 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature