M⁶ A demethylase ALKBH5 drives denervation-induced muscle atrophy by targeting HDAC4 to activate FoxO3 signalling.

ABSTRACT:

Background

Skeletal muscle atrophy is a common clinical manifestation of various neurotrauma and neurological diseases. In addition to the treatment of primary neuropathies, it is a clinical condition that should be investigated. FoxO3 activation is an indispensable mechanism in denervation-induced muscle atrophy; however, upstream factors that control FoxO3 expression and activity have not been fully elucidated. N⁶ -methyladenosine (m⁶ A) methylation is a novel mode of epitranscriptional gene regulation that affects several cellular processes. However, the biological significance of m⁶ A modification in FoxO3-dependent atrophy is unknown.

Methods

We performed gain-of-function and loss-of-function experiments and used denervation-induced musc

SUBMITTER: Liu Y 

PROVIDER: S-EPMC8978003 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature