Cathepsin K activity controls cachexia-induced muscle atrophy via the modulation of IRS1 ubiquitination.

ABSTRACT:

Background

Cachexia is a complicated metabolic disorder that is characterize by progressive atrophy of skeletal muscle. Cathepsin K (CTSK) is a widely expressed cysteine protease that has garnered attention because of its enzymatic and non-enzymatic functions in signalling in various pathological conditions. Here, we examined whether CTSK participates in cancer-induced skeletal muscle loss and dysfunction, focusing on protein metabolic imbalance.

Methods

Male 9-week-old wild-type (CTSK^+/+ , n = 10) and CTSK-knockout (CTSK^-/- , n = 10) mice were injected subcutaneously with Lewis lung carcinoma cells (LLC; 5 × 10⁵ ) or saline, respectively. The mice were then subjected to muscle mass and muscle function measurements. HE staining, immunostainin

SUBMITTER: Meng X 

PROVIDER: S-EPMC8978007 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature