Establishment of a controlled slow freezing-based approach for experimental clinical cryopreservation of human prepubertal testicular tissues.

ABSTRACT:

Objective

To develop an efficient, clinical-grade, freezing protocol toward experimental clinical cryopreservation of testicular tissues in prepubertal boys suffering from cancer.

Design

Experimental cryopreservation of testicular tissue.

Setting

University Medical Center.

Patients

Adult patients undergoing orchiectomy for various tumors and prepubertal boys scheduled for gonadotoxic treatment.

Interventions

None.

Main outcome measures

Histopathological analysis of tissue architecture, structural integrity, and cellular morphology was performed for control and frozen-thawed cryopreserved tissues.The number of seminiferous tubules per testicular section was calculated. The survival of spermatogonial stem cells (SSCs) and Sertoli cells of the contro

SUBMITTER: Kabiri D 

PROVIDER: S-EPMC8978083 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature