Esophageal Microbiota and Nutritional Intakes in Patients With Achalasia Before and After Peroral Endoscopic Myotomy.

ABSTRACT:

Background/aims

The composition of the microbiota in the esophagus is only partially understood, especially in patients with achalasia. We aim to investigate the esophageal microbial community and nutritional intakes in patients with achalasia before and after peroral endoscopic myotomies (POEM).

Methods

Twenty-nine patients were prospectively enrolled from 4 referral institutions across Korea. We collected esophageal samples (mucosal biopsies and retention fluid) and conducted dietary surveys for nutritional intake before and 8 weeks after POEM. The esophageal microbiota was analyzed by 16S rRNA gene sequencing targeting the V3-V4 region.

Results

Out of the 105 samples from 29 patients, 99 samples were subjected to microbial bioinformatic analysis after quality contr

SUBMITTER: Jung DH 

PROVIDER: S-EPMC8978113 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature