Complete sequence of classic F-type plasmid pRK100 shows unique conservation over time and geographic location.

ABSTRACT: Plasmids exhibit great diversity of gene content and host ranges and are famous for quick adaptation to the genetic background of the bacterial host cell. In addition to observing ever evolving plasmids, some plasmids have conserved backbones: a stable core composition and arrangement of genes in addition to variable regions. There are a few reports of extremely conserved plasmids. Here we report the complete sequence of pRK100 plasmid - a large, well-characterized conjugative F-like plasmid found in an Escherichia coli strain isolated from a urinary tract infection patient in 1990. The sequence shows that the 142 kb-long pRK100 plasmid is nearly identical to plasmids circulating in distant geographical locations and found in different host E. coli strains between 2007 and 2017. We also pe

SUBMITTER: Starcic Erjavec M 

PROVIDER: S-EPMC8978152 | biostudies-literature | 2022 Jan-Mar

REPOSITORIES: biostudies-literature