The HipAB Toxin-Antitoxin System Stabilizes a Composite Genomic Island in Shewanella putrefaciens CN-32.

ABSTRACT: Composite genomic islands (GIs) are useful models for studying GI evolution if they can revert into the previous components. In this study, CGI48-a 48,135-bp native composite GI that carries GI21, whose homologies specifically integrated in the conserved yicC gene-were identified in Shewanella putrefaciens CN-32. CGI48 was integrated into the tRNA^Trp gene, which is a conserved gene locus for the integration of genomic islands in Shewanella. Upon expressing integrase and excisionase, CGI48 and GI21 are excised from chromosomes via site-specific recombination. The shorter attachment sites of GI21 facilitated the capture of GI21 into CGI48. Moreover, GI21 encodes a functional HipAB toxin-antitoxin system, thus contributing to the maintenance of CGI48 in

SUBMITTER: Zhao Y 

PROVIDER: S-EPMC8978831 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature