Perspective: Emerging strategies for determining atomic-resolution structures of macromolecular complexes within cells.

ABSTRACT: In principle, electron cryo-tomography (cryo-ET) of thin portions of cells provides high-resolution images of the three-dimensional spatial arrangement of all members of the proteome. In practice, however, radiation damage creates a tension between recording images at many different tilt angles, but at correspondingly reduced exposure levels, versus limiting the number of tilt angles in order to improve the signal-to-noise ratio (SNR). Either way, it is challenging to read the available information out at the level of atomic structure. Here, we first review work that explores the optimal strategy for data collection, which currently seems to favor the use of a limited angular range for tilting the sample or even the use of a single image to record the high-resolution information. Looking t

SUBMITTER: Petrov PN 

PROVIDER: S-EPMC8978977 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature