Turn-on signal fluorescence sensor based on DNA derived bio-dots/polydopamine nanoparticles for the detection of glutathione.

ABSTRACT: A convenient, fast, sensitive and highly selective fluorescence sensor for the detection of glutathione (GSH) based on DNA derived bio-dots (DNA bio-dots)/polydopamine (PDA) nanoparticles was constructed. The fluorescent switch of DNA bio-dots was induced to turn off because of fluorescence resonance energy transfer (FRET) reactions between DNA bio-dots and PDA. The presence of GSH blocked the spontaneous oxidative polymerization of dopamine (DA) to PDA, leading the fluorescent switch of DNA bio-dots to be "turned on". The degree of fluorescence recovery of DNA bio-dots is linearly correlated with the concentration of GSH within the range of 1.00-100 μmol L^-1, and the limit of detection (LOD) is 0.31 μmol L^-1 (S/N = 3, n = 9). Furthermore, the fluorescence sens

SUBMITTER: Chen X 

PROVIDER: S-EPMC8979005 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature