NF-κB perturbation reveals unique immunomodulatory functions in Prx1⁺ fibroblasts that promote development of atopic dermatitis.

ABSTRACT: Skin is composed of diverse cell populations that cooperatively maintain homeostasis. Up-regulation of the nuclear factor κB (NF-κB) pathway may lead to the development of chronic inflammatory disorders of the skin, but its role during the early events remains unclear. Through analysis of single-cell RNA sequencing data via iterative random forest leave one out prediction, an explainable artificial intelligence method, we identified an immunoregulatory role for a unique paired related homeobox-1 (Prx1)⁺ fibroblast subpopulation. Disruption of Ikkb-NF-κB under homeostatic conditions in these fibroblasts paradoxically induced skin inflammation due to the overexpression of C-C motif chemokine ligand 11 (CCL11; or eotaxin-1) characterized by eosinophil infiltration and a subs

SUBMITTER: Ko KI 

PROVIDER: S-EPMC8979241 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature