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Deactivation of a dimeric DNA-binding peptide through a palladium-mediated self-immolative cleavage.

ABSTRACT: Herein, we describe an approach for the on-demand disassembly of dimeric peptides using a palladium-mediated cleavage of a designed self-immolative linker. The utility of the strategy is demonstrated for the case of dimeric basic regions of bZIP transcription factors. While the dimer binds designed DNA sequences with good affinities, the peptide-DNA complex can be readily dismounted by addition of palladium reagents that trigger the cleavage of the spacer, and the release of unfunctional monomeric peptides.

SUBMITTER: Rodriguez J

PROVIDER: S-EPMC8979313 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature

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Deactivation of a dimeric DNA-binding peptide through a palladium-mediated self-immolative cleavage.

Rodríguez Jessica J Pérez-González Cibrán C Martínez-Calvo Miguel M Mosquera Jesús J Mascareñas José L JL

RSC advances 20220126 6

Herein, we describe an approach for the on-demand disassembly of dimeric peptides using a palladium-mediated cleavage of a designed self-immolative linker. The utility of the strategy is demonstrated for the case of dimeric basic regions of <i>b</i>ZIP transcription factors. While the dimer binds designed DNA sequences with good affinities, the peptide-DNA complex can be readily dismounted by addition of palladium reagents that trigger the cleavage of the spacer, and the release of unfunctional ...[more]

PMID: 35425354

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