Identification of cis-regulatory modules for adeno-associated virus-based cell-type-specific targeting in the retina and brain.

ABSTRACT: Adeno-associated viruses (AAVs) targeting specific cell types are powerful tools for studying distinct cell types in the central nervous system (CNS). Cis-regulatory modules (CRMs), e.g., enhancers, are highly cell-type-specific and can be integrated into AAVs to render cell type specificity. Chromatin accessibility has been commonly used to nominate CRMs, which have then been incorporated into AAVs and tested for cell type specificity in the CNS. However, chromatin accessibility data alone cannot accurately annotate active CRMs, as many chromatin-accessible CRMs are not active and fail to drive gene expression in vivo. Using available large-scale datasets on chromatin accessibility, such as those published by the ENCODE project, here we explored strategies to increase efficiency in identi

SUBMITTER: Lin CH 

PROVIDER: S-EPMC8980332 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature