Human induced pluripotent stem cells integrate, create synapses and extend long axons after spinal cord injury.

ABSTRACT: Numerous interventions have been explored in animal models using cells differentiated from human induced pluripotent stem cells (iPSCs) in the context of neural injury with some success. Our work seeks to transplant cells that are generated from hiPSCs into regionally specific spinal neural progenitor cells (sNPCs) utilizing a novel accelerated differentiation protocol designed for clinical translation. We chose a xenotransplantation model because our laboratory is focused on the behaviour of human cells in order to bring this potential therapy to translation. Cells were transplanted into adult immunodeficient rats after moderate contusion spinal cord injury (SCI). Twelve weeks later, cells derived from the transplanted sNPCs survived and differentiated into neurons and glia that filled th

SUBMITTER: Lavoie NS 

PROVIDER: S-EPMC8980929 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature