Untargeted 2D NMR Metabolomics of [¹³C-methyl]Methionine-Labeled Tumor Models Reveals the Non-DNA Methylome and Provides Clues to Methyl Metabolism Shift during Tumor Progression.

ABSTRACT: For more than a decade, DNA and histone methylations have been the focus of extensive work, although their relationship with methyl group metabolism was overlooked. Recently, it has emerged that epigenetic methylations are influenced by methyl donor nutrient availability, cellular levels of S-adenosyl-methionine (SAM), and cytoplasmic methyltransferase activities. SAM-dependent methyltransferases methylate a wide range of targets, from small molecules to proteins and nucleic acids. However, few investigations of the global methylome of tumors have been performed. Here, untargeted NMR metabolomics of two mouse tumor models labeled with [¹³C-methyl]methionine were used to search for the NMR-visible set of cellular methyl acceptors denoted the global methylome. Tumor

SUBMITTER: Morvan D 

PROVIDER: S-EPMC8981308 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature