Protection of the Prodomain α1-Helix Correlates with Latency in the Transforming Growth Factor-β Family.

ABSTRACT: The 33 members of the transforming growth factor beta (TGF-β) family are fundamentally important for organismal development and homeostasis. Family members are synthesized and secreted as pro-complexes of non-covalently associated prodomains and growth factors (GF). Pro-complexes from a subset of family members are latent and require activation steps to release the GF for signaling. Why some members are latent while others are non-latent is incompletely understood, particularly because of large family diversity. Here, we have examined representative family members in negative stain electron microscopy (nsEM) and hydrogen deuterium exchange (HDX) to identify features that differentiate latent from non-latent members. nsEM showed three overall pro-complex conformations that differed in prodo

SUBMITTER: Le VQ 

PROVIDER: S-EPMC8981510 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature