Downregulation of KRAB zinc finger proteins in 5-fluorouracil resistant colorectal cancer cells.

ABSTRACT: Radio-chemotherapy with 5-flu orouracil (5-FU) is the standard of care treatment for patients with colorectal cancer, but it is only effective for a third of them. Despite our understanding of the mechanism of action of 5-FU, drug resistance remains a significant limitation to the clinical use of 5-FU, as both intrinsic and acquired chemoresistance represents the major obstacles for the success of 5-FU-based chemotherapy. In order to identify the mechanism of acquired resistance, 5-FU chemoresistance was induced in CRC cell lines by passaging cells with increasing concentrations of 5-FU. To study global molecular changes, quantitative proteomics and transcriptomics analyses were performed on these cell lines, comparing the resistant cells as well as the effect of chemo and radiotherapy. In

SUBMITTER: Chauvin A 

PROVIDER: S-EPMC8981854 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature