Excitation-emission matrix fluorescence spectroscopy for cell viability testing in UV-treated cell culture.

ABSTRACT: Monitoring of cells viability is essential in a number of biomedical applications, including cell-based sensors, cell-based microsystems, and cell-based assays. The use of spectroscopic techniques for such purposes is especially advantageous since they are non-invasive, label-free, and non-destructive. However, such an approach must include chemometric analysis of the data to assess the information on cells viability. In the presented article we demonstrate, that excitation-emission matrix (EEM) fluorescence spectroscopy can be applied for reliable determination of cells viability due to the high correlation of EEM fluorescence data with the MTT test data. A375 cells (malignant melanoma) were exposed to UV radiation as a physical stress factor, resulting in a decrease of viability up to

SUBMITTER: Glowacz K 

PROVIDER: S-EPMC8982211 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature