Bur1 functions with TORC1 for vacuole-mediated cell cycle progression.

ABSTRACT: The vacuole/lysosome plays essential roles in the growth and proliferation of many eukaryotic cells via the activation of target of rapamycin complex 1 (TORC1). Moreover, the yeast vacuole/lysosome is necessary for progression of the cell division cycle, in part via signaling through the TORC1 pathway. Here, we show that an essential cyclin-dependent kinase, Bur1, plays a critical role in cell cycle progression in cooperation with TORC1. A mutation in BUR1 combined with a defect in vacuole inheritance shows a synthetic growth defect. Importantly, the double mutant, as well as a bur1-267 mutant on its own, has a severe defect in cell cycle progression from G₁ phase. In further support that BUR1 functions with TORC1, mutation of bur1 alone results in high sensitivity to rapamycin,

SUBMITTER: Jin Y 

PROVIDER: S-EPMC8982600 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature