Spatial and temporal control of mitochondrial H2 O2 release in intact human cells.
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ABSTRACT: Hydrogen peroxide (H2 O2 ) has key signaling roles at physiological levels, while causing molecular damage at elevated concentrations. H2 O2 production by mitochondria is implicated in regulating processes inside and outside these organelles. However, it remains unclear whether and how mitochondria in intact cells release H2 O2 . Here, we employed a genetically encoded high-affinity H2 O2 sensor, HyPer7, in mammalian tissue culture cells to investigate different modes of mitochondrial H2 O2 release. We found substantial heterogeneity of HyPer7 dynamics between individual cells. We further observed mitochondria-released H2 O2 directly at the surface of the orga
SUBMITTER: Hoehne MN
PROVIDER: S-EPMC8982624 | biostudies-literature | 2022 Apr
REPOSITORIES: biostudies-literature
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