Exosomal miR-543 Inhibits the Proliferation of Ovarian Cancer by Targeting IGF2.

ABSTRACT:

Objective

Ovarian cancer (OvCa) is the most lethal gynaecological malignancy worldwide. We aimed to illustrate the potential function and molecular mechanism of exosomal microRNA-543 (miR-543) in the oncogenesis and development of OvCa.

Methods

Differentially expressed microRNAs in exosomes derived from OvCa cell lines were identified by bioinformatic analysis and verified by RT-PCR. Cell proliferation ability was estimated by clonogenic and 5-ethynyl-2'-deoxyuridine assays in vitro and in vivo. Potential involved pathways and targets of exosomal miRNAs were analysed using DIANA and verified by pyrosequencing, glucose quantification, dual-luciferase reporter experiments, and functional rescue assays.

Results

Bioinformatic analysis identified miR-543 and its potential

SUBMITTER: Zhang S 

PROVIDER: S-EPMC8983272 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature