Participation of Histones in DNA Damage and Repair within Nucleosome Core Particles: Mechanism and Applications.

ABSTRACT: DNA is damaged by various endogenous and exogenous sources, leading to a diverse group of reactive intermediates that yield a complex mixture of products. The initially formed products are often metastable and can react to yield lesions that are more biologically deleterious. Mechanistic studies are frequently carried out on free DNA as the substrate. The observations do not necessarily reflect the reaction environment inside human cells where genomic DNA is condensed as chromatin in the nucleus. Chromatin is made up of monomeric structural units called nucleosomes, which are comprised of DNA wrapped around an octameric core of histone proteins (two copies each of histones H2A, H2B, H3, and H4).This account presents a summary of our work in the past decade on the mechanistic studies of DNA

SUBMITTER: Ren M 

PROVIDER: S-EPMC8983524 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature