PA1 cells containing a truncated DNA polymerase β protein are more sensitive to gamma radiation.

ABSTRACT:

Purpose

DNA polymerase β (Polβ) acts in the base excision repair (BER) pathway. Mutations in DNA polymerase β (Polβ) are associated with different cancers. A variant of Polβ with a 97 amino acid deletion (PolβΔ), in heterozygous conditions with wild-type Polβ, was identified in sporadic ovarian tumor samples. This study aims to evaluate the gamma radiation sensitivity of PolβΔ for possible target therapy in ovarian cancer treatment.

Materials and methods

PolβΔ cDNA was cloned in a GFP vector and transfected in PA1 cells. Stable cells (PA1PolβΔ) were treated with 60Co sourced gamma-ray (0-15 Gy) to investigate their radiation sensitivity. The affinity of PolβΔ with DNA evaluated by DNA protein in silico docking experiments.

Results

The result showed a statistically sig

SUBMITTER: Patra A 

PROVIDER: S-EPMC8984132 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature