Precore/core mutations of hepatitis B virus genotype D arising in different states of infection.

ABSTRACT:

Aim of the study

Precore/core variations and liver disease progression have been suggested. In this study, we aimed to determine the frequency of precore/core mutations in hepatitis B virus (HBV)-infected patients at various clinical stages.

Material and methods

In total, 73 HBV-infected patients including 26 inactive carriers (IC), 20 chronic active (CA), and 27 patients with liver cirrhosis/hepatocellular carcinoma (C/HCC) were randomly selected. The HBV DNA was extracted from the sera and subjected to nested PCR for amplification of precore/core region. The PCR product was then sequenced by the Sanger method.

Results

The stop codon of W28*(G1896A) was determined as the most prevalent mutation (55%) of the precore region. The comparison of groups also demonstrated t

SUBMITTER: Sanaei N 

PROVIDER: S-EPMC8984791 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature