Detection of Salivary Tryptase Levels in Children following Oral Food Challenges.

ABSTRACT:

Background

Oral food challenge (OFC) is commonly used to diagnose food allergy. This test is time and resource intensive, and conclusions are not always unequivocal as this relies on the interpretation of symptoms. Therefore, an objective marker would improve the accuracy of the diagnostic workup of food allergy.

Objectives

The aim of this study was to investigate whether tryptase can be detected in saliva of children following OFC.

Method

Children from 3 to 18 years of age were eligible for inclusion if an OFC for peanut or tree nut had been recommended. Saliva samples were collected prior to the first dose and 5, 10, and 15 min following the last administered dose during OFC. Assay precision, spike-and-recovery, and assessment of lower limit of detection of the tryp

SUBMITTER: de Weger WW 

PROVIDER: S-EPMC8985013 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature