Identification and functional analysis of cation-efflux transporter 1 from Brassica juncea L.

ABSTRACT:

Background

Brassica juncea behaves as a moderate-level accumulator of various heavy metal ions and is frequently used for remediation. To investigate the roles of metal ion transporters in B. juncea, a cation-efflux family gene, BjCET1, was cloned and functionally characterized.

Results

BjCET1 contains 382 amino acid residues, including a signature motif of the cation diffusion facilitator protein family, six classic trans-membrane-spanning structures and a cation-efflux domain. A phylogenetic analysis showed that BjCET1 has a high similarity level with metal tolerance proteins from other Brassica plants, indicating that this protein family is highly conserved in Brassica. BjCET1 expression significantly increased at very early stages during both cadmium and zinc treatments.

SUBMITTER: Han L 

PROVIDER: S-EPMC8985314 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature