Single-cell transcriptomics of the ventral posterolateral nucleus-enriched thalamic regions from HSV-1-infected mice reveal a novel microglia/microglia-like transcriptional response.

ABSTRACT:

Background

Microglia participate in the immune response upon central nervous system (CNS) infections. However, the role of these cells during herpes simplex encephalitis (HSE) has not been fully characterized. We sought to identify different microglia/microglia-like cells and describe the potential mechanisms and signaling pathways involved during HSE.

Methods

The transcriptional response of CD11b⁺ immune cells, including microglia/microglia-like cells, was investigated using single-cell RNA sequencing (scRNA-seq) on cells isolated from the ventral posterolateral nucleus (VPL)-enriched thalamic regions of C57BL/6 N mice intranasally infected with herpes simplex virus-1 (HSV-1) (6 × 10⁵ PFUs/20 µl). We further performed scanning electronic microscopy (SE

SUBMITTER: Uyar O 

PROVIDER: S-EPMC8985399 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature