Semi-rational design and molecular dynamics simulations study of the thermostability enhancement of cellobiose 2-epimerases.

ABSTRACT: Directed evolution using random mutation in vast sequence space leads to the low probability of obtaining target proteins. Emerging engineering strategies with computational tools are developed for more trustable outcomes. We used some semi-rational design methods to modify an industrial enzyme, namely cellobiose 2-epimerase (CE). A mutant was selected for its better thermostability and isomerization activity. The tradeoffs between thermostability, epimerization activity and isomerization activity of the CE mutants were different. To investigate the computational prediction performance of protein stability upon point mutations, molecular dynamics (MD) simulation analyses were conducted. The root mean square deviation (RMSD) and hydrogen bond analyses reproduced the correct trends in stabil

SUBMITTER: Chen Q 

PROVIDER: S-EPMC8985420 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature