Expressions of IL4, IL10, and IFNγ cytokines genes during bacterial mastitis.

ABSTRACT:

Objective

Many bacteria are involved in causing mastitis in dairy cows. Perfect identification of bacteria is crucial for the appropriate choice of drug for treatment. This study aims to find out the various bacteria that cause mastitis through the 16S ribosomal ribonucleic acid (16S rRNA) gene.

Materials and methods

A total of 150 mastitis somatic cell samples were tested with bacterial nested polymerase chain reaction (PCR) universal primers, targeting the 16S rRNA gene. The primers had both Gram-positive and Gram-negative bacterial specificities. Inflammatory cytokine interleukin (IL-10), IL-4, and interferon-gamma (IFNγ) expression genes were measured and compared in mastitis-free and mastitis-affected animals.

Results

Based on the PCR, 70 (46.7%) sa

SUBMITTER: Faaz RA 

PROVIDER: S-EPMC8985890 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature