Glyco-Steroidal Amphiphiles (GSAs) for Membrane Protein Structural Study.

ABSTRACT: Integral membrane proteins pose considerable challenges to high resolution structural analysis. Maintaining membrane proteins in their native state during protein isolation is essential for structural study of these bio-macromolecules. Detergents are the most commonly used amphiphilic compounds for stabilizing membrane proteins in solution outside a lipid bilayer. We previously introduced a glyco-diosgenin (GDN) detergent that was shown to be highly effective at stabilizing a wide range of membrane proteins. This steroidal detergent has additionally gained attention due to its compatibility with membrane protein structure study via cryo-EM. However, synthetic inconvenience limits widespread use of GDN in membrane protein study. To improve its synthetic accessibility and to further enhance

SUBMITTER: Ehsan M 

PROVIDER: S-EPMC8986615 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature