3D holotomographic monitoring of Ca⁺⁺ dynamics during ionophore-induced Neospora caninum tachyzoite egress from primary bovine host endothelial cells.

ABSTRACT: Neospora caninum represents an obligate intracellular parasite that belongs to the phylum Apicomplexa and is a major abortive agent in bovines. During merogony, N. caninum tachyzoites invade and proliferate in host cells in vivo, including endothelial cells of lymphatic and blood vessels. The egress at the end of the lytic cycle is tightly regulated in apicomplexans. Evidence in Toxoplasma gondii shows that Ca⁺⁺ signalling governs tachyzoite egress. Much less is known on egress mechanisms of N. caninum. Here, we show, using 3D live cell holotomographic microscopy in fluo-4 AM-loaded N. caninum-infected BUVEC, that treatments with the calcium ionophore A23187 at 24- and 42-h post-infection (h p. i.) induced a fast and sustained increase in Ca⁺⁺ signals in parallel to t

SUBMITTER: Larrazabal C 

PROVIDER: S-EPMC8986705 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature