Extracellular Vesicles From Microalgae: Uptake Studies in Human Cells and Caenorhabditis elegans.

ABSTRACT: Extracellular vesicles (EVs) are lipid membrane nano-sized vesicles secreted by various cell types for intercellular communication, found in all kingdoms of life. Nanoalgosomes are a subtype of EVs derived from microalgae with a sustainable biotechnological potential. To explore the uptake, distribution and persistence of nanoalgosomes in cells and living organisms, we separated them from a culture of the chlorophyte Tetraselmis chuii cells by tangential flow filtration (TFF), labelled them with different lipophilic dyes and characterized their biophysical attributes. Then we studied the cellular uptake of labelled nanoalgosomes in human cells and in C. elegans, demonstrating that they enter the cells through an energy dependent mechanism and are localized in the cytoplasm of

SUBMITTER: Picciotto S 

PROVIDER: S-EPMC8987914 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature