Small molecule LATS kinase inhibitors block the Hippo signaling pathway and promote cell growth under 3D culture conditions.

ABSTRACT: Although 3D cell culture models are considered to reflect the physiological microenvironment and exhibit high concordance with in vivo conditions, one disadvantage has been that cell proliferation is slower in 3D culture as compared to 2D culture. However, the signaling differences that lead to this slower proliferation are unclear. Here, we conducted a cell-based high-throughput screening study and identified novel small molecules that promote cell proliferation, particularly under 3D conditions. We found that one of these molecules, designated GA-017, increases the number and size of spheroids of various cell-types in both scaffold-based and scaffold-independent cultures. In addition, GA-017 also enhances the ex vivo formation of mouse intestinal organoids. Importantly, we demonstrate th

SUBMITTER: Aihara A 

PROVIDER: S-EPMC8988011 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature