Characterization of fecal microbiome in biopsy positive prostate cancer patients.

ABSTRACT:

Objectives

To characterize the fecal microbiome in newly diagnosed prostate cancer patients.

Patients and methods

Forty-nine consecutive patients who were referred for trans rectal prostate biopsy were tested. Patients who received antibiotics 3 months prior to the biopsy, patients with history of pelvic irradiation, prostate or colon cancer, inflammatory bowel disease and urinary tract infection were excluded. A rectal swab was obtained just prior to the biopsy, immediately placed in a sterile tube and kept in -80°C. Sequencing was performed for the 16S rRNA 515F + 806R gene fragment using the QIIME2 software. Analytic tests included Beta diversity (Weighted Unifrac, Unweighted Unifrac, Bray-Curtis), Alpha diversity (Faith, Evenness), Taxa bar plots and PCoA plots.

Resul

SUBMITTER: Katz R 

PROVIDER: S-EPMC8988526 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature