TRPV4 channels mediate the mechanoresponse in retinal microglia.

ABSTRACT: The physiological and neurological correlates of plummeting brain osmolality during edema, traumatic CNS injury, and severe ischemia are compounded by neuroinflammation. Using multiple approaches, we investigated how retinal microglia respond to challenges mediated by increases in strain, osmotic gradients, and agonists of the stretch-activated cation channel TRPV4. Dissociated and intact microglia were TRPV4-immunoreactive and responded to the selective agonist GSK1016790A and substrate stretch with altered motility and elevations in intracellular calcium ([Ca²⁺ ]_i ). Agonist- and hypotonicity-induced swelling was associated with a nonselective outwardly rectifying cation current, increased [Ca²⁺ ]_i , and retraction of higher-order processes. Th

SUBMITTER: Redmon SN 

PROVIDER: S-EPMC8989051 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature