Kymolyzer, a Semi-Autonomous Kymography Tool to Analyze Intracellular Motility.

ABSTRACT: The movement of intracellular cargo, such as transcripts, proteins, and organelles, is fundamental to cellular function. Neurons, due to their long axons and dendrites, are particularly dependent on proper intracellular trafficking and vulnerable to defects in the movement of intracellular cargo that are noted in neurodegenerative and neurodevelopmental disorders. Accurate quantification of intracellular transport is therefore needed for studying the mechanisms of cargo trafficking, the influence of mutations, and the effects of potentially therapeutic pharmaceuticals. In this article, we introduce an algorithm called "Kymolyzer." The algorithm can quantify intracellular trafficking along a defined path, such as that formed by the aligned microtubules of axons and dendrites. Kymolyzer work

SUBMITTER: Basu H 

PROVIDER: S-EPMC8989283 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature