Hepatocellular carcinoma-infiltrating γδ T cells are functionally defected and allogenic Vδ2⁺ γδ T cell can be a promising complement.

ABSTRACT: In hepatocellular carcinoma (HCC), γδ T cells participate in mediating the anti-tumour response and are linked with a positive prognosis. However, these cells can become pro-tumoural in the tumour microenvironment (TME). We aimed to decipher the immune landscape and functional states of HCC-infiltrating γδ T cells to provide fundamental evidence for the adoptive transfer of allogeneic Vδ2⁺ γδ T cells in HCC immunotherapy. We performed single-cell RNA sequencing (scRNA-seq) on γδ T cells derived from HCC tumours and healthy donor livers. Confocal microscopy, flow cytometry and a Luminex assay were applied to validate the scRNA-seq findings. The γδ T cells in the HCC TME entered G2/M cell cycle arrest, and expressed cytotoxic molecules such as interferon-gamma and granzyme B, but

SUBMITTER: He W 

PROVIDER: S-EPMC8989380 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature