Global chromosome rearrangement induced by CRISPR-Cas9 reshapes the genome and transcriptome of human cells.

ABSTRACT: Chromosome rearrangement plays important roles in development, carcinogenesis and evolution. However, its mechanism and subsequent effects are not fully understood. Large-scale chromosome rearrangement has been performed in the simple eukaryote, wine yeast, but the relative research in mammalian cells remains at the level of individual chromosome rearrangement due to technical limitations. In this study, we used CRISPR-Cas9 to target the highly repetitive human endogenous retrotransposons, LINE-1 and Alu, resulting in a large number of DNA double-strand breaks in the chromosomes. While this operation killed the majority of the cells, we eventually obtained live cell groups. Karyotype analysis and genome re-sequencing proved that we have achieved global chromosome rearrangement (GCR) in hum

SUBMITTER: Liu Y 

PROVIDER: S-EPMC8989517 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature