Summarizing internal dynamics boosts differential analysis and functional interpretation of super enhancers.

ABSTRACT: Super enhancers (SEs) are broad enhancer domains usually containing multiple constituent enhancers that hold elevated activities in gene regulation. Disruption in one or more constituent enhancers causes aberrant SE activities that lead to gene dysregulation in diseases. To quantify SE aberrations, differential analysis is performed to compare SE activities between cell conditions. The state-of-art strategy in estimating differential SEs relies on overall activities and neglect the changes in length and structure of SEs. Here, we propose a novel computational method to identify differential SEs by weighting the combinatorial effects of constituent-enhancer activities and locations (i.e. internal dynamics). In addition to overall activity changes, our method identified four novel classes of

SUBMITTER: Liu X 

PROVIDER: S-EPMC8989535 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature